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α1 2 fucose vectorlabs b  (Vector Laboratories)


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    Vector Laboratories α1 2 fucose vectorlabs b
    α1 2 Fucose Vectorlabs B, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 472 article reviews
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    AGP glycosylation pattern in lectin staining. 2-DE (IPG pH-range 2.5-5) under reducing conditions; blot onto nitrocellulose. Animals: A: adult animal (female, 7 months); a: neonatal piglet (1d); sample: serum, 2.5fold volume of adult. Stains: Ab: A , a : anti-human AGP (Dako, Glostrup, Denmark). Lectins: B , b : concanavalin A (Con A, specific for α-mannose); C , c : Sambuccus nigra bark lectin (SNA, SNL/EBL, specific for sialic acid α(2-6) linked to galactose); D , d : Aleuria aurantia lectin (AAL, specific for α(1-6)-linked fucose); blots were incubated with biotinylated lectins (Vector Laboratories, Burlingame, CA) and biotinylation detected by NeutrAvidin-HRPO (Pierce Biotechnologies, Rockford, IL); HRPO was detected by ECL on a Vilber Lourmat FX system (Vilber-Lourmat, Eberhardzell, Germany)

    Journal: Veterinary Research Communications

    Article Title: Proteomic methods for the study of porcine acute phase proteins – anything new to detect?

    doi: 10.1007/s11259-023-10170-6

    Figure Lengend Snippet: AGP glycosylation pattern in lectin staining. 2-DE (IPG pH-range 2.5-5) under reducing conditions; blot onto nitrocellulose. Animals: A: adult animal (female, 7 months); a: neonatal piglet (1d); sample: serum, 2.5fold volume of adult. Stains: Ab: A , a : anti-human AGP (Dako, Glostrup, Denmark). Lectins: B , b : concanavalin A (Con A, specific for α-mannose); C , c : Sambuccus nigra bark lectin (SNA, SNL/EBL, specific for sialic acid α(2-6) linked to galactose); D , d : Aleuria aurantia lectin (AAL, specific for α(1-6)-linked fucose); blots were incubated with biotinylated lectins (Vector Laboratories, Burlingame, CA) and biotinylation detected by NeutrAvidin-HRPO (Pierce Biotechnologies, Rockford, IL); HRPO was detected by ECL on a Vilber Lourmat FX system (Vilber-Lourmat, Eberhardzell, Germany)

    Article Snippet: Lectins: B , b : concanavalin A (Con A, specific for α-mannose); C , c : Sambuccus nigra bark lectin (SNA, SNL/EBL, specific for sialic acid α(2-6) linked to galactose); D , d : Aleuria aurantia lectin (AAL, specific for α(1-6)-linked fucose); blots were incubated with biotinylated lectins (Vector Laboratories, Burlingame, CA) and biotinylation detected by NeutrAvidin-HRPO (Pierce Biotechnologies, Rockford, IL); HRPO was detected by ECL on a Vilber Lourmat FX system (Vilber-Lourmat, Eberhardzell, Germany) Also HPT is glycosylated but, in contrast to AGP and as already mentioned earlier, the glycan residues on the molecule in the cited study were not changed in infection (Marco-Ramell et al. ).

    Techniques: Glycoproteomics, Staining, Incubation, Plasmid Preparation